FileNotFoundError: [Error 2] No such file or directory: '......'

The following is the code:

from Bio import SeqIO
fasta_sequences = SeqIO.parse(open(’~/Desktop/basedOnGISAID/spikeprot0105.fasta’),‘fasta’)

And then, on the title of the Atom window, following the python icon, it says

genFasta.py – ~/Desktop
When I run the code, I got “FileNotFoundError”, while the file is indeed there. Could anyone explain a bit to me about this?

The file ‘spikeprot0105.fasta’ has 466MB in size. Is it because the file “spikeprot0105.fasta” too large?